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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Neuropharmacology. 2017 Oct 16;128:255–268. doi: 10.1016/j.neuropharm.2017.10.014

Figure 8. DAMGO, but not CTX MVIIC, decreases NMDA-induced [Ca2+]i responses in DRG neurons.

Figure 8

A. Cultured DRG neurons were loaded for 1 h with 5 μM Fura-2 AM and then incubated for 15 min with 20 ng/ml BDNF with or without 1 μM DAMGO. Responses were evoked by rapid infusion of 250 μM NMDA + 10 μM glycine. B. DRG neurons were loaded for 1 h with Fura-2 AM without (control) or with 1 μM CTX GVIA (CTX), and then incubated with BDNF as above before stimulation with 250 μM NMDA + 10 μM glycine. C. Neurons loaded with Fura-2 AM for 1 h with or without CTX GVIA were stimulated with 50 mM KCl to induce the opening of Cav channels. Bars show the median response and the interquartile range. A Mann-Whitney test was used to obtain the p values given in each panel.