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. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: J Am Chem Soc. 2016 Jul 1;138(27):8497–8504. doi: 10.1021/jacs.6b03599

Figure 1.

Figure 1

Schematic representation of the nanoprobe-based detection of the highly mutagenic O6-MeG. The novel synthetic nucleosides ExBenzi and ExBIM lead to the formation of more stable DNA duplexes when they are paired with O6-MeG than with G. Gold nanoparticles (AuNPs) functionalized with ordinary oligonucleotides serve as detection nanoprobes while AuNPs functionalized with oligonucleotides containing a synthetic nucleoside (indicated by terminal P) serve as discriminating nanoprobes. In the presence of O6-MeG target DNA strands, the detection nanoprobes can align in a tail-to-tail fashion with the discriminating nanoprobes. The hybridization of the target leads to aggregation of AuNPs accompanied by a change in the color of the solution from red to purple.