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. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: J Am Chem Soc. 2016 Jul 1;138(27):8497–8504. doi: 10.1021/jacs.6b03599

Table 1.

Melting temperatures of 13-mer DNA duplexes centered on KRAS codon 13 in which the target strand contains in the middle position either G or O6-MeG and the base in the middle position of the complementary strand is C, ExBenzi, or ExBIM.

Paired bases Tm(°C) ΔTm(°C)a
C:G
C:O6-MeG		 66.0±0.6
55.2±0.2		 -10.8±0.6
ExBenzi:G
ExBenzi: O6-MeG		 52.0±0.7
55.0±0.7		 +3.0±1.0
ExBIM:G
ExBIM: O6-MeG		 51.7±0.6
54.0±1.0		 +2.3±1.2
a

ΔTm= Tm(O6-MeG duplex)- Tm(G duplex)

b

Data are mean ± SD from three independent experiments