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Changes in temperature and pacing rate do not alter apelin (10 nM) effects on contractile parameters in freely contracting cells, seeded on a non-stick surface. A) Contractile response to apelin in isolated, field stimulated wild-type murine ventricular myocytes at different temperatures (N = 12 at 37 ± 2 °C and 6 at 24 ± 2 °C, n > 40 per condition). B) Contractile response to apelin at different pacing rates (N = 12 at 1 Hz and 6 at 2 Hz, n > 40 per condition). Unpaired experiments.
