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. 2017 Dec 12;12(12):e0189528. doi: 10.1371/journal.pone.0189528

Fig 2. The inhibitory effects of CSS are independent of the presence of sex and thyroid hormones.

Fig 2

ECs were incubated in Standard Medium (SM, solid bars), CSS-medium (CSS-M, open bars), CSS-M + E2 (1 nM, spotted bars), CSS-M + DHT (10 nM, diagonal bars), or CSS-M + T3 (10 nM, squared bars). MTT absorbance (A, C, E) or cell number (B, D, F) were measured after 48h of incubation. Cell number was expressed as percent of control, i.e. cells cultured in SM, set at 100%. In (A), (C), and (E), *p<0.05, **p<0.01 (CSS-M vs SM), ns (CSS-M vs CSS-M + E2, DHT or T3), n = 3, t test. In (B), (D), and (F), *p<0.05, **p<0.01, ***p<0.001 (CSS-M vs SM), ns (CSS-M vs CSS-M + E2, DHT or T3), n = 3, t test. (G) CSS-M was replaced with CSS-M (open bars) or SM (diagonal bars), and MTT was measured after further 48h of incubation. Solid bars: SM replaced with SM. ***p<0.001 vs SM, °°°p<0.001 vs CSS-M, n = 3, t test. F- and M-ECs are orange and blue, respectively.