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. 2017 Dec 12;12(12):e0189528. doi: 10.1371/journal.pone.0189528

Fig 6. Palmitic acid and acetate rescue the CSS-induced inhibition of in vitro angiogenesis.

Fig 6

(A) Representative images of spheroids from F-ECs incubated for 24 h in SM, CSS-M, CSS-M + palmitic acid, or CSS-M + acetate. The cumulative (B, D) and the average (C, E) length of sprouts emerging from F-ECs (B, C) and M-ECs (D, E) were measured after 24h of incubation. Treatments and bars are as in Fig 5: SM, solid bars; CSS + vehicle (ethanol/BSA, cross-hitched bars), CSS + palmitic acid (250 μM, vertical bars); CSS-M, open bars; CSS-M + acetate (20 mM, horizontal bars). Data are expressed as percent of control, i.e. the cumulative and the average length of sprouts from cells cultured in SM, set at 100%. In (B) and (D), ***p<0.001 (CSS-M + vehicle vs SM), *p<0.05, **p<0.01 (CSS-M + vehicle vs CSS-M + palmitic acid), n = 3; °°°p<0.001 (CSS-M vs SM), °p<0.05 (CSS-M vs CSS-M + acetate), n = 4, t test. In (C) and (E), **p<0.01 (CSS-M + vehicle vs SM), *p<0.05, ***p<0.001 (CSS-M + vehicle vs CSS-M + palmitic acid), n = 3; °p<0.05, °°°p<0.001 (CSS-M vs SM), °°p<0.01, °°°p<0.001 (CSS-M vs CSS-M + acetate), n = 4, t test. F- and M-ECs are orange and blue, respectively.