Strips 1 and 2 correspond with serum samples from dogs considered negative for both TBRF and Lyme disease (LD). Strips 3 and 4: correspond to dogs with positive serology for TBRF and negative serology for LD. Strip 5: corresponds to a dog with negative serology for TBRF and positive serology for LD. Strip 6: corresponds to a dog with positive serology for TBRF and LD. TBRF/LD Seology refers to the combined result of ELISA and Immunoblot assay for both diseases. Strip No. Refers to the provided numbers by the manufacturer. IC refers to the internal Controls on the strip. 93, 66, 60, 58, 45, 41, 39, 34, 31, 30, 28, 23 and 18 refers to the molecular weight in kDa of each B. burgdorferi antigen immobilized on the strip. Numbers in red refer to bands that lack specificity (cross-reactive) while the green numbers correspond with antigens used for the diagnostic of LD. Gray numbers correspond with antigens that have certain cross reactivity between TBRF and LD observed during this study.