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(A) SEM images showing hMSCs spheroid morphology, (B) cell integrity and viability staining (blue dots; scale bar = 50 μm), (C) typical 3D structure (x20, Z-stacked image of Hoechst 33342 stained nuclei) and (D) comparative analysis of spheroid size at P2, 3 to 4. (Mean ± SD; n = 12).
