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. 2017 Dec 12;12(12):e0188882. doi: 10.1371/journal.pone.0188882

Fig 4. CXCR4 blockade decreased the percentage of PD-1 expressing adaptive immune cells in sepsis.

Fig 4

(A-B) Representative flow plots (gated on CD3 cells) demonstrating PD-1 expression in CD4+ T cells and CD8+ T cells. (C) The frequency of PD-1+ CD4+ T cells was significantly increased in septic mice compared to sham mice (28.3% vs. 16.8%; p = 0.002). When septic mice were treated with plerixafor, the frequency of PD-1+ CD4+ T cells was significantly decreased compared to septic control mice (21.1% vs. 28.3%; p = 0.0156). (D) In septic mice treated with plerixafor, the per-cell expression of PD-1 on CD4+ T cells, as measured by MFI, was significantly decreased compared to septic control mice (66.1 vs. 76.8; p = 0.033). (E-F) There was no statistically significant difference in frequency (E) or MFI (F) of PD-1+ CD8+ T cells in septic mice treated with plerixafor compared to septic control mice (21.5% vs. 30.0%; p = 0.169 and 58.0 vs. 68.3; p = 0.327, respectively). N = 4–8 mice/group. Representative of 3 independent experiments with a total of 12–24 mice/group.