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. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: Exp Cell Res. 2017 Jun 30;358(2):253–259. doi: 10.1016/j.yexcr.2017.06.022

Fig. 1.

Fig. 1

PWS measurement of Hela Cells at different stages of fixation. The higher value (redder) indicates higher level of mass-density heterogeneity. (A) (C) (E) show live Hela cells without any treatment. (B) After 4% PFA fixation. (D) After 95% EtOH fixation. (F)–(H), after 2.5% GA and 2% FA, after 1% OsO4, and after serial ethanol dehydration. Color scale: (A) (B) (E) (F) from 0.005 to 0.15, (D) from 0.005 to 0.5, (G) and (H) from 0.05 to 0.25. The inset squares are higher magnification images of nucleus and cytoplasm region.