. Author manuscript; available in PMC: 2018 Nov 1.

Published in final edited form as: J Cardiovasc Pharmacol. 2017 Nov;70(5):314–328. doi: 10.1097/FJC.0000000000000524

Table 2.

Modulation of respiration rate in isolated cardiac mitochondria after cardiac IR injury ±BK_Ca + SK_Ca and/or SK_Ca channel antagonists.

Pyruvate	state 2	state 3	state 4	RCI
TC	6.8 ±0.7	210.2 ±22.4	11.6 ±3.8	18.1 ±2.5
IR	35.7 ±2.3^§	112.0 ±33.9^§	59.4 ±3.4^§	2.2 ±2.0^§
IR+PAX	12.0 ±2.6^*^§	92.3 ±25.2^§	53.4 ±3.6^§	1.7 ±1.9^§
IR+NS8593	30.9 ±2.1^*^§	145.1 ±37.0^§	58.6 ±6.2^§	2.3 ±3.0^§
IR+PAX+NS8593	20.6 ±1.1^*^§	96.0 ±20.1^§	59.4 ±3.7^§	1.6 ±0.8^§
Succinate	state 2	state 3	state 4	RCI

TC	64.5 ±0.8	450.4 ±37.5	79.5 ±10.3	5.7 ±0.5
IR	72.0 ±3.2^§	220.2 ±32.9^§	129.0 ±12.0^§	1.7 ±1.2^§
IR+PAX	90.1 ±6.1^*^§	286.2 ±38.8^§	154.1 ±14.2^§	1.8 ±1.1^§
IR+NS8593	168.1 ±8.2^*^§	280.0 ±53.3^§	161.1 ±18.2^§	1.7 ±0.8^§
IR+PAX+NS8593	116.1 ±9.5^*^§	246.0 ±38.5^§	178.2 ±15.2^*^§	1.4 ±0.8^§
Succinate+rotenone	state 2	state 3	state 4	RCI

TC	62.4 ± 9.8	290.5 ±56.2	76.8 ±0.8	3.8 ±0.6
IR	87.2 ± 6.4	202.0 ±32.9	105.2 ±16.4^§	1.9 ±0.7^§
IR+PAX	77.4 ±11.6	234.2 ±55.1	136.0 ±33.7^§	1.7 ±0.8^§
IR+NS8593	96.2 ±14.2^§	312.0 ±48.6^*	261.3 ±38.2^*^§	1.2 ±0.8^§
IR+PAX+NS8593	108.2 ±26.0^§	305.3 ±45.7^*	262.3 ±31.6^*^§	1.2 ±0.7^§

Respiration rate (O₂ consumption, nmol/min/mg protein) was measured in cardiac mitochondria isolated at 20 min perfusion after 35 min global ischemia ± BK_Ca and SK_Ca channel antagonists NS8593 ± PAX given 20 min before and for 20 min after ischemia. RCI (respiratory control index) = state 3/state 4. Time controls (TC) were not subject to ischemia but 75 min perfusion. Note that the lower RCI’s after IR were not significantly lower after hearts were treated with PAX ± NS8593 compared to IR alone with any substrate. N = hearts/group with 3–4 replicates within each mitochondrial pellet. P<0.05;

IR + drugs vs. IR only;

^§

IR ± drugs vs. TC.