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. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: Cell Rep. 2017 Nov 14;21(7):1953–1967. doi: 10.1016/j.celrep.2017.10.021

Figure 4. Melanoma PDX metastasize spontaneously.

Figure 4

(A) Animals were grafted with neonatal foreskin grafts and melanoma PDX cells were injected into established grafts. (B) Melanoma lesions formed in the human skin reconstructs. (C) Melanomas spontaneously metastasized to the mouse lungs from the human skin graft. H&E staining, and representative images. (D) Example of spontaneous micro-metastasis to lung. (E) Percentage of PDX that metastasize to lungs in more than 80% of animals from the subcutaneous tumor graft at the time point of maximal tumor volume. (F) Number of PDX with spontaneous lung metastasis compared to main mutational subgroups. (G) Luciferase transfected brain metastasis PDX injected s.c.. (H) Spontaneous metastases to the mouse brain were imaged ex vivo after a latency of 120 days after survival surgery. (I) Percentage of IGF1R positive cells in PDX from naïve patients, from patients progressed on BRAF inhibitor (−BR), on BRAF inhibitor or BRAF/MEKi combination diet.