Skip to main content
. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: Cell Rep. 2017 Nov 28;21(9):2571–2584. doi: 10.1016/j.celrep.2017.10.118

Figure 3. Impaired intestinal regeneration by conditional ablation of Tert+ cells.

Figure 3

(A) Illustration of conditional ablation of Tert+ cells (TertTCE/+:R26DTA). 4OHT treatment activates TCE, which leads to the expression of diphtheria toxin A (DTA) for Tert+ cell ablation.

(B) Scheme of mouse treatment.

(C–K) Impairment of intestinal regeneration by Tert+ cell ablation. H&E staining (C); cytokeratin 19 (CK19) (D); Ki67 (E); lysozyme (Paneth cell, H); PAS (goblet cell, I); villin (enterocyte, J); chromogranin A (ChgA; enteroendocrine cell, K). Of note, Tam-treated TertTCE/+:R26DTA did not affect intestinal homeostasis (Figures S3B–S3I). Quantification of the number of Ki67+ cells (F) and CC3+ cells (G) per 500 μm region of crypts. Scale bars=20μm; dot lines indicate the basal membranes below crypts.

The representative images are shown; N≥3.