Figure 4. The att, fasR, and fas loci are necessary for the pathogenicity of Rhodococcus.

(A) Representative images of leafy galls on N. benthamiana. Isolates of Rhodococcus were inoculated at the apical meristem. Red arrows indicate the leafy galls. Rs114, Rs23, and Rs29 are abbreviations for isolates 114MFTsu3.1, UNC23MFCrub1.1, and 29MFTsu3.1, respectively. (B) Representative images of the root length of seedlings. Three-day-old N. benthamiana seedlings were inoculated with theindicated isolate of Rhodococcus or water (mock) and grown vertically for seven days under constant light. Isolates D188, A44a, A21d2, and A25f are the only isolates with virulence genes .(C) Quantification of seedling root length. All treatments, except for PBTS2, were significant compared to the mock treatment.