(
A) RNAs used in the study (same as (
A) in
Figure 3, shown again here for convenience). (
B–C) Observed rates (k
obs) min
−1 of mRNA recruitment as a function of the concentration of eIF4A. Data were fit with a hyperbolic equation allowing for a y-intercept >0 (see Materials and methods). Numbers in parentheses, to the right of the coordinate plane, correspond to mRNAs in (
A) and are colored for easier visualization of distinct curves. RNAs 2 and 3 are shown separately for clarity. (
D) Expansion of plots for mRNAs 8 and 9 from (
B) for clarity. (
E) The observed rate of recruitment in the absence of eIF4A (
), the maximal rate of recruitment with saturating eIF4A (k
max), and the concentration of eIF4A required to achieve the half-maximal rate of recruitment (
) from fits in panels B-D. ‘ND':’ the observed rate from an exponential fit could not be determined because of low reaction endpoint. All data presented in the figure are mean values (n ≥ 2) and error values are average deviation of the mean.