Skip to main content
. Author manuscript; available in PMC: 2018 May 27.
Published in final edited form as: Nat Chem Biol. 2017 Nov 27;14(1):65–71. doi: 10.1038/nchembio.2520

Figure 1. Effects of fucose analogs on Notch signaling in Zebrafish embryos.

Figure 1

(a) Numbered structures of GDP-fucose analogs screened as potential inhibitors of Notch signaling in Zebrafish. (b) Peracetylated fucose analogs selected for further analysis in cell-based assays. See Supplementary Figure 1a for structures of other peracetylated fucose analogs. (c) Transgenic Zebrafish embryos expressing a GFP fluorescent Notch signaling reporter showed that some injected GDP-fucose analogs, indicated in each panel, reduced Notch signaling. Knock down of GDP-mannose-4,6-dehydratase (gmds MO) to inhibit endogenous GDP-fucose biosynthesis was used as a positive control for the effect of eliminating Notch O-fucosylation and Notch signaling. Scale bar represents 0.5 mm.