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. Author manuscript; available in PMC: 2018 May 27.
Published in final edited form as: Nat Chem Biol. 2017 Nov 27;14(1):65–71. doi: 10.1038/nchembio.2520

Figure 2. Peracetylated fucose analogs are efficiently incorporated into Notch EGF repeats and elongated by Lfng.

Figure 2

EGF1–18-MycHis from mNotch1 was transfected into HEK293T cells expressing no Fng (a–c) or Lfng (d–f) and the cells were grown in the presence of compounds 9 (a, d), 10 (b, e), or 11 (c, f) for 72 hours. Purified EGF1–18-MycHis was digested and subjected to nano-LC-MS/MS analysis. (a–c) Extracted ion chromatograms (EIC) of the ions corresponding to glycoforms (see key) of this peptide show efficient incorporation of the corresponding compounds. (d–f) Similar experiments in the presence of Lfng demonstrate elongation of fucose analogs with GlcNAc. See Supplementary Figure 2 for corresponding mass spectra and Supplementary Note 1 for m/z ratios used to generate EICs.