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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Colloids Surf B Biointerfaces. 2017 Oct 23;161:200–209. doi: 10.1016/j.colsurfb.2017.10.060

Figure 1. Preparation of antibody-coated MBs.

Figure 1

A) Conjugation of Traut’s reagent-modified antibody (red residue) to preformed, perflorohexane gas-filled (blue), PEG-maleimide-decorated MBs; B) Formulations F0 and F1 that showed the best stability upon preparation were selected for Ab conjugation (full list of tested formulations is in Supplemental Table S2); C) Storage stability of MBs at 4°C for 7 days after preparation. Antibody decorated MBs (F0A and F1A) show better stability than the original formulations as evidenced by less coalescence and foaming of MBs; D) size (diameter) distribution of MBs; E) anti-EpCAM/EGFR MBs, anti-EpCAM MBs and anti-EGFR MBs were stained with secondary antibodies (anti-human IgG for anti-EpCAM, anti-mouse IgG for anti-EGFR and both antibodies for anti-EGFR/EpCAM and control MBs). Images confirm that MBs were decorated with one or both antibody types. Note that anti-EGFR MBs have much weaker fluorescence because only a small part of cetuximab is of murine origin. Size bar is 15 μm for all samples.