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. 2017 Dec 8;11:100. doi: 10.3389/fncir.2017.00100

Figure 1.

Figure 1

Cholinergic responses in adult human neocortex are cell type and lamina-dependent. (A) Left: example reconstruction of biocytin-labeled human L2/3 pyramidal neuron. Right: No change in membrane potential occurred in response to a local application (puff) of 1 mM Acetylcholine (Ach; green bar, 30 s). (B) Left: example reconstruction of biocytin-labeled human L6 pyramidal neuron. Right: examples of no change in membrane potential (top) and a depolarization (bottom) in response to a local application of 1 mM ACh (green bar, 30 s). (C) Examples of biocytin-labeled human interneurons, with action potential firing profiles in response to step current injections and voltage or current responses to local application of 1 mM ACh (green bar, 30 s). Gray traces were recorded in the presence of nicotinic acetylcholine receptor (nAChR) blockers mecamylamine (MEC, middle traces, 1 μM) or dihydro-b-erythroidine hydrobromide (DHβE, bottom traces, 1 μM). (D) Overview of functional nicotinic AChR expression in pyramidal neurons and the predicted expression in interneurons in different lamina of the human temporal cortex. Data figures are representing edited versions of previously published findings (Alkondon et al., 2000; Verhoog et al., 2016).