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. 2017 Dec 12;7:17415. doi: 10.1038/s41598-017-17694-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multiple active site conformations in A3Bctd crystal structures. (A) A tightly closed conformation as observed previously in the crystal structure of A3B(187–378)QM_Δloop3¹⁵. (B) The fully open conformation as observed in the crystal structure of an A3Bctd variant bound to ssDNA²⁵. (C) A-chain of A3B(187–378)QM_Δloop3 in the new crystal form. (D) B-chain of A3B(187–378)QM_Δloop3 in the new crystal form. The gray spheres represent the zinc ion in the active site.