Figure 1.

The complementing YFP Venus fusion proteins are correctly targeted to the ER and mitochondria. NSC 34 cells transiently expressing V1-VAPB (A), V1-ER (B), or V2-Mito (C), were analysed by immunocytochemistry using an anti-GFP antibody that recognises both fragments of YFP. The endoplasmic reticulum was identifed by co-expression of ER-DsRed2 (Clontech), and mitochondria were labelled immunolcytochemically for ATPB. The extent of colocalisation of Venus fusion proteins with the orgamelle markers was analysed by Pearon’s Correlation Coefficient (PCC) (Table 1). Values indicate mean PCC ± SEM; n = 3 independent experiments (9–10 cells total). Antibody details Table 3. Scale bars, 10 μm.