Table 3.
Differential presence of genes involved in biofilm synthesis and glycerophospholipid catabolism, with special relevance to biofilm formation in A. sobria.
| Category | RAST annotation | Major product/function | A. sobria | |||
|---|---|---|---|---|---|---|
| TM12 | TM18 | JF2635 | CECT 4245 | |||
| Biofilm adhesin biosynthesis | pgaA (Biofilm PGA outer membrane secretin) | Outer membrane PGA | P | P | ||
| pgaC [Biofilm PGA synthesis N-glycosyl transferase (EC 2.4.-.-)] | GlcNAc export to periplasm | P | P | |||
| pgaB (Biofilm PGA synthesis deacetylase (EC 3.-)) | Promotes PGA export through the PgaA porin | P | P | |||
| Glycero- phospholipid Catabolism | Phospholipase C 4 precursor (EC 3.1.4.3) | DAG∗ + phospholipid head group | P | P | ||
| Putative phospholipase A1-like (EC 3.1.1.32) | Lysophospholipid∗ + free fatty acid | P | P | P | P | |
| Lysophospholipase L2 (EC 3.1.1.5) | Free fatty acid | P | P | P | ||
| Biofilm formation in LB | + | ++ | - | ++ | ||
| Biofilm formation in TSB | + | - | - | - | ||
PGA, Poly-beta-1,6-N-acetyl-D-glucosamine. DAG, diacylglycerol. GlcNAc, N-acetyl glucosamine. EC, Enzyme Commission number for enzymes. P, present. +, weak biofilm formation. ++, strong biofilm formation. -, no biofilm production. ∗known surfactants.