Figure 7.

Transfer of peritoneal macrophages overexpressing QKI5 rescued lipopolysaccharide (LPS)-induced endotoxic shock. (A) Peritoneal macrophages overexpressing QKI5 (2 × 10⁶/mouse) or control cells (LV-Cherry) were intraperitoneally injected 0.5 h after QKI^fl/fl and LysMCre QKI^fl/fl mice were challenged with LPS (40 mg/kg). Survival was observed. The data shown are pooled from the two independent experiments (n = 20). (B,C) Serum samples were collected 4 h after LPS injection. Inflammatory cytokines TNF-α, IL-6, IL-1β (B), and IL-10 (C) were analyzed using the Dakawe mouse enzyme-linked immunosorbent assay (ELISA) assay kit. (D) Twenty-four hours after LPS challenge, mouse peritoneal cells were collected and analyzed by flow cytometry to discriminate macrophage subtypes. F4/80^highCD11b^high (M2) and F4/80^intCD11b^int (M1) gated out of total live cells. (E) Quantification of M1 and M2 cells in (D) obtained from LPS-challenged mice. All animal experiments were performed twice, independently and each time 10 mice/group were used in (A–C). n = 20. For (D), 6–9 mice/group were used. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.