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. 2017 Dec 8;8:2050. doi: 10.3389/fpls.2017.02050

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Copyright © 2017 Huck, Leissing, Majovsky, Buntru, Aretz, Flecken, Müller, Vogel, Schillberg, Hoehenwarter, Conrath and Beckers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative analysis of the intensity ratios of labeled and unlabeled peptide pairs. (A) Mass spectra of the phosphopeptide LAAPSpSPFDDDSDDVDEQPLVR mapping to AT4G11740 (SAY1) in tandemMOAC extracted samples of reciprocally¹⁴N- and ¹⁵N-labeled seedlings treated with ethanol as a control (-EST) or with β-estradiol (+EST). m/z represents the ratio of ion mass over the charge of each phosphopeptide ion. (B) Same as in (A) but spectra correspond to the VVLKPVSHpSPK phosphopeptide of AT1G62390 (CLMP1). NL, normalized intensity level (counts per second).