Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 8;8:2050. doi: 10.3389/fpls.2017.02050

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Huck, Leissing, Majovsky, Buntru, Aretz, Flecken, Müller, Vogel, Schillberg, Hoehenwarter, Conrath and Beckers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

In vitro thiophosphorylation assay of MPK3/6. (A) Thiophosphorylation assays of GST-tagged wild type (Wt)- and analog-sensitive (AS)-MPK3 in the presence of Bn-ATPγS as cofactor. Tested StrepII-tagged substrates (SUB, e.g., eYFP, VQ4, HON5, DRP2B, WRKY6, CLMP1, MKK2, and NOT2/3/5) were purified from cell-free in vitro transcription-translation reactions using tobacco BY-2 cell lysates. Western blotting analysis, and immunodetection with specific antibodies was performed to check equal amounts of kinase (α-GST) and substrate (α-STREP) in each reaction and to examine phosphorylation of substrates (α-TPE). (B) Same as in (A) but using Wt- and AS-MPK6.