Table 1.
Identified and quantified in vivo substrate candidate proteins of the MKK7-MPK3/6 module.
| ATG | Protein description | Peptide sequence |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ratio EST +/- (sample 1–8) |
Score | m z-1 |
z |
|||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Mean ratio | p-value | |||
| AT1G62390 | CLUMPED CHLOROPLASTS 1 (CLMP1) | (1) VVLKPVSHpSPK | 44 | 424.2 | 3 | |||||||
| 4.1 | 3.6 | 5.1 | 3.3 | 3.0 | 4.9 | 3.2 | 2.9 | 3.8 | 5.25E-07 | |||
| AT1G28280 | VQ MOTIF-CONTAINING PROTEIN (VQ4) | (2) LLPLFPVTpSPR | 46 | 660.4 | 2 | |||||||
| 5.9 | 6.8 | 3.5 | 5.4 | 4.6 | 4.4 | 4.6 | 5.0 | 1.05E-06 | ||||
| AT1G62300 | WRKY6 | (3) LGREEpSPETESNKIQK | 39 | 642.3 | 3 | |||||||
| 6.7 | 7.6 | 6.6 | 17.0 | 3.8 | 3.5 | 4.2 | 5.5 | 6.9 | 2.09E-05 | |||
| (8) LGREEpSPETESNK | 52 | 787.3 | 2 | |||||||||
| 12.0 | 4.7 | 12.6 | 9.2 | 6.6 | 9.0 | 3.27E-04 | ||||||
| AT1G10290 | DYNAMIN-RELATED PROTEIN 2A (DRP2A) | (4) AAAASSYSDNSGTESpSPR | 92 | 919.4 | 2 | |||||||
| 2.4 | 1.6 | 2.1 | 1.9 | 1.8 | 2.2 | 2.0 | 8.05E-05 | |||||
| AT4G38710 | GLYCINE-RICH PROTEIN | (5) TLPVAVVEVVKPEpSPVLVIVEKPK | 87 | 892.8 | 3 | |||||||
| 12.3 | 4.2 | 5.6 | 3.4 | 3.6 | 20.5 | 4.0 | 7.4 | 7.6 | 9.34E-05 | |||
| AT1G07110 | ”FRUCTOSE-2.6-BISPHOSPHATASE” (F2KP) | (6) SVETLpSPFQQK | 45 | 672.3 | 2 | |||||||
| 3.7 | 3.9 | 4.9 | 4.1 | 4.2 | 1.93E-04 | |||||||
| AT4G29810 | MAP KINASE KINASE 2 (MKK2) | (7) IISQLEPEVLpSPIKPADDQLSLSDLDMVK | 59 | 1,091.9 | 3 | |||||||
| 1.9 | 1.8 | 2.6 | 1.5 | 1.3 | 2.3 | 1.7 | 1.4 | 1.8 | 2.71E-04 | |||
| AT1G59610 | DYNAMIN-RELATED PROTEIN 2B (DRP2B) | (9) AAAASSWSDNSGTESpSPR | 86 | 930.9 | 2 | |||||||
| 2.6 | 2.4 | 2.1 | 2.4 | 2.4 | 3.49E-04 | |||||||
| AT5G18230 | TRANSCRIPTION REGULATOR NOT2/NOT3/NOT5 FAMILY PROTEIN | (10) NIMGVESNVQPLTpSPLSK | 88 | 997.5 | 2 | |||||||
| 7.9 | 25.4 | 11.0 | 41.2 | 21.7 | 21.5 | 6.08E-04 | ||||||
| AT4G38550 | Arabidopsis PHOSPHOLIPASE (pEARLI4)-LIKE PROTEIN | (11) NSSPPpSPFHPAAYK | 66 | 790.4 | 2 | |||||||
| 4.6 | 8.2 | 3.0 | 5.8 | 4.7 | 2.1 | 4.7 | 6.66E-04 | |||||
| (16) STPGSPAHPPGARpSPPPSYLSNK | 42 | 794.7 | 3 | |||||||||
| 4.1 | 1.7 | 3.9 | 3.3 | 0.8 | 2.8 | 4.71E-02 | ||||||
| AT1G48620 | HIGH MOBILITY GROUP A5 (HON5) | (12) KDGTpSPTVKPAASVSGGVETVK | 37 | 740.7 | 3 | |||||||
| 2.0 | 1.8 | 2.1 | 1.4 | 1.8 | 6.30E-03 | |||||||
| AT4G15545 | UNCHARACTERIZED PROTEIN | (13) HSSIQSQQASEAIEPAATDNENDAPKPSLSASLPLVSQTTpTPR | 50 | 1,139.3 | 4 | |||||||
| 1.3 | 1.7 | 1.2 | 1.9 | 1.4 | 1.5 | 7.19E-03 | ||||||
| AT1G27100 | ACTIN CROSS-LINKING PROTEIN | (14) RPTSSPLSAEpSPR | 50 | 732.8 | 2 | |||||||
| 7.4 | 10.4 | 12.2 | 1.8 | 8.0 | 2.30E-02 | |||||||
| AT5G12850 | ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 56 | (15) TLNPSNLEELFSAEVApSPR | 92 | 1,077.5 | 2 | |||||||
| 3.1 | 2.4 | 5.6 | 1.3 | 11.6 | 4.8 | 2.49E-02 | ||||||
In addition to the calculated mean ratio, for each peptide β-estradiol-to-control ratios (EST +/–) are provided separately for each of the eight samples. Capital letters in the peptide sequence column indicate amino acids and lower case p indicates phosphorylation of the following S/T-P phosphorylation motif. Mean ratios and corresponding p-values are highlighted in bold. EST, β-estradiol; m z-1, mass over charge.