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. 2017 Dec 12;7:17403. doi: 10.1038/s41598-017-16540-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TRPP2-mediated acceleration of cytoskeletal inhibition is not mediated by a faster recruitment of F-actin filaments. Averaged traces showing the recovery of LifeAct-RFP fluorescence signal following photobleaching at t = 1.5 s in COS7 cells expressing TRPP2 (n = 34) or Mock (n = 24) plasmids at 37 °C (a) and 22 °C (b). Respective mean ( ± s.e.m.) recovery time constants at 37 °C (c) and at 22 °C (d). (e) Averaged traces showing the recovery of GFP-Actin signal following photobleaching at t = 1.5 s in COS7 expressing TRPP2 (n = 21) or Mock (n = 21) plasmids at 22 °C with respective (f) mean ( ± s.e.m.) recovery time constant and (g) mean (±s.e.m.) mobile fraction. No significant differences were observed using an unpaired t test.