Skip to main content
. 2017 Dec 8;10:402. doi: 10.3389/fnmol.2017.00402

FIGURE 2.

FIGURE 2

FRRS1L is localized at dynein, but not kinesin, vesicles in hippocampus. (A) The schematic of vesicle membrane preparation from detergent-free hippocampal homogenate. (B) Differential distributions of FRRS1L, γ8, CNIH2, and the AMPAR (probed by a pan-AMPAR antibody) in 8/35, 35/40, and post-ultracentrifugation PNS membrane fractions (PNSM) (N = 4 independent repeats). (C,D) Vesicle immunoisolation of 8/35 membrane fraction by a dynein (C, the antibody was against the dynein intermediate chain, DIC) or kinesin (D, the antibody was against the KIF5B) antibodies. The 10% input was also included. The immunoprecipitates were mixed with SDS-PAGE sample buffer and subjected to Western blotting by indicated antibodies (N = 5 independent repeats). Uncropped scans of Western blots in Supplementary Figures 2B–D.