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. 2017 Dec 12;7:17471. doi: 10.1038/s41598-017-17803-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Newcastle disease virus (NDV) mini-genome expression of nucleoprotein (NP) mutants. The NDV mini-genome expression of full-length NDV NP was compared with NP truncation mutants (A,B) and NP deletion mutants (C,D). (A and C) Schematic representation of full-length NDV NP-Myc and NP-Myc mutants. (B and D) Mini-genome RNA synthesis activity of the NP mutants. BSR T7 cells were transfected with plasmids as described in the Materials and Methods. The relative luciferase expression levels of cells transfected with plasmids encoding NP-Myc were defined as 100%. Expression of NP-Myc and NP-Myc mutants was detected via western blotting using an anti-Myc antibody. The gels are uncropped images. Asterisks indicate a statistically significant difference between the titers of the chimeric virus and the parental virus. P-values were calculated based on a two-tailed, unpaired t-test (95% confidence levels). *P = 0.01–0.05; **P = 0.001–0.01; ***P = 0.0001–0.001. n = 3.