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. 2017 Dec 12;7:17471. doi: 10.1038/s41598-017-17803-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nucleoprotein (NP) interacts with phosphoprotein (P) independent of the E402 site. 293 T cells were co-transfected with 2.5 μg of plasmids expressing HA-tagged P protein, together with 2.5 μg of plasmids expressing Flag-tagged NP or NP_E402A. At 48 h post-transfection, cells were harvested and immunoprecipitated with anti-Flag M2 beads. The precipitates were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and P as well as NP proteins were detected in immunoblots using anti-HA or anti-Flag antibodies. The gels are uncropped images.