Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 12;8:2094. doi: 10.1038/s41467-017-02350-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Csn2 impacts NHEJ repair. Survival rates of irradiated B. subtilis cells. a–c Individual replicates (points) and average (horizontal bars) are shown. Error bars correspond to the standard error of the mean (s.e.m.). a Cells carrying the type II-A CRISPR-Cas system (pRH87) or the control empty vector (pC194), and deleted for ku and ligD or not (P = 0.0009, Wilcoxon's). b B. subtilis carrying the CRISPR-Cas system with the dCas9 mutations (pRH121) or deleted for csn2 (pRH63), cas1 (pRH61), or cas2 (pRH62) (P = 0.02, Student's two-sided t test after validation of normality and homoscedasticity). c B. subtilis carrying the empty pC194 plasmid (Ø), expressing csn2 from plasmid pAB56 or cas9 from plasmid pDB114 (P = 0.0048, Wilcoxon's). d A linearized plasmid providing resistance to chloramphenicol (pC194) was electroporated into S. aureus RN4220 cells carrying the NHEJ system either alone (plasmid pAB1, Ø) or with csn2 cloned downstream of ligD (plasmid pAB81, csn2) or under the control of its natural promoter (plasmid pAB82, csn2 n.p.). The number of CFUs obtained with or without induction of the NHEJ system using aTc are reported. The number of CFU obtained without induction (gray bars) indicate the background of already circular DNA present in the sample before electroporation (P = 0.006, two-sided t test)