Fig. 5.

Comparison of BrS patient and control hiPSC-CM differentiation and APs.

A–B) qRT-PCR analysis of marker expression during cardiac cluster differentiation of control iPS-HS1M and BrS patient iBR1-P5M-L1 lines. Data displayed from n = 3 (A) or n = 4 (B) independent differentiation experiments ± s.e.m. C–G) APs recorded from isolated iPS-HS1M (n = 21) and iBR1-P5M-L1 CMs (n = 17) at weeks 6–8 of differentiation. C) Representative traces and proportion of AP types (assigned as described in Methods). The arrow heads indicate that the AP was triggered (at 1 Hz). The grey dashed line indicates 0 mV. D–G) Collated AP data displaying the range of APD₉₀ values (D), Resting Membrane Potential (RMP) (E), AP amplitude (F) and Upstroke Velocity (G). Data displayed as mean ± s.e.m. * = p ≤ 0.05 (independent t-test) vs control line iPS-HS1M.