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. 2017 Dec 12;7:17366. doi: 10.1038/s41598-017-17644-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Light microscopy of L-form cultures from cord blood samples. (A) Development of pure L-form culture in broth after filtration through 0.2 µm bacterial filter. Clusters of spherical L-form cells with different size - native smear (CB6). (B) ZN stained smear from a broth culture. Arrows point to red stained rods, acid fast mycobacteria reverted from L-forms after further sub-cultivation in broth (CB6). (C) L-form colonies with “fried eggs” shape after sub-cultivation in semisolid medium. (D) Tiny L-form “fried eggs” colonies after sub-cultivation in semisolid medium (CB10). Magnification: A, B - 1000x; C, D- 400x.