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. 2017 Dec 12;7:17429. doi: 10.1038/s41598-017-17653-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nod2 is involved in early clearance of A. baumannii from lungs. Wild type (WT) or Nod2^−/− mice were infected with A. baumannii (n = 4–6 mice per group). At designated time points bacterial cfu was enumerated in lungs as described in material and methods and the bacterial load in lungs was compared between the 2 groups at 4 (a and c), 12 (b), and 24 h (a) post infection. The confirmed bacterial inoculation doses were 1 × 10⁷ (a and b) and 2 × 10⁷ (c) respectively. **Represents p ≤ 0.01, ***represents p ≤ 0.001, ****represents p ≤ 0.0001. Values shown represent mean ± standard deviation of results of three independent experiments.