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. 2017 Dec 12;7:17375. doi: 10.1038/s41598-017-17388-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Device Working Principle: (a) Image of the sorting device to control the z-position of the particles as they enter the ridged channel using vertical sheath. H and Hf are the heights of the channel and focused streamline respectively. (b) COMSOL simulations for different sample to sheath flow rate ratio (f). As the vertical sheath flow rate increases the sample flow streamlines are pushed down. (c) COMSOL simulations show the velocity field under the ridge and the lateral deviation of streamlines as they enter the ridged part of channel at different heights. (d) Cross-stream velocity distribution.