Table 1.
Human Blood-Nerve Barrier Transcriptome validation antibodies.
| Gene Symbol | Protein Name | In Vitro FPKM | In Situ FPKM | Antibody Source | Catalog Number | Host/Isotype | Final Concentration |
|---|---|---|---|---|---|---|---|
| ABCA8 | Lipid transporter | 0.4 | 119.3 | Life Technologies | PA5-60866 | Rabbit polyclonal IgG | 2 µg/mL |
| ABCB1 | P-glycoprotein | 2.3 | 23.0 | Life Technologies | MA5-13854 | Mouse IgG1 | 4 µg/mL |
| ACTG1 | Gamma 1 actin | 3012.4 | 242.7 | Life Technologies | PA5-13467 | Rabbit polyclonal IgG | 40 µg/mL |
| AQP1 | Aquaporin 1 | 1.68 | 29.0 | Santa Cruz Biotechnology | sc-25287 | Mouse IgG1 (kappa light chain) | 4 µg/mL |
| CALD1 | Caldesmon 1 | 140.6 | 750.5 | Abcam | ab68878 | Rabbit polyclonal IgG | 1 µg/mL |
| CAV1 | Caveolin 1 | 681.7 | 43.0 | Santa Cruz Biotechnology | sc-53564 | Mouse IgG2b (kappa light chain) | 4 µg/mL |
| CD44 | Cell surface adhesion glycoprotein | 120.0 | 50.1 | Santa Cruz Biotechnology | sc-7297 | Mouse IgG1 (kappa light chain) | 4 µg/mL |
| CD63 | Tetraspanin family member glycoprotein | 1132.9 | 33.1 | Santa Cruz Biotechnology | sc-5275 | Mouse IgG2a (kappa light chain) | 4 µg/mL |
| CDH5 | Cadherin 5 | 340.1 | 19.1 | Santa Cruz Biotechnology | sc-9989 | Mouse IgG1 (kappa light chain) | 4 µg/mL |
| CDH6 | Cadherin 6 | 1.8 | 7.2 | Life Technologies | MA1-06305 | Mouse IgG1 | 20 µg/mL |
| CLDN4 | Claudin 4 | 0.9 | 2.5 | Life Technologies | 36-4800 | Rabbit polyclonal IgG | 2.5 µg/mL |
| CLDN5 | Claudin 5 | 362.1 | 17.7 | Life Technologies | 35-2500 | Mouse IgG1 | 10 µg/mL |
| CTGF | Connective tissue growth factor | 709.0 | 44.6 | Santa Cruz Biotechnology | sc-101586 | Mouse IgG1 | 2 µg/mL |
| CTNNA1 | Alpha 1 catenin | 158.6 | 218.7 | Life Technologies | 13-9700 | Mouse IgG1 (kappa light chain) | 2 µg/mL |
| CXCR4 | CXC chemokine receptor 4 | 263.8 | 3.4 | Santa Cruz Biotechnology | sc-53534 | Mouse IgG1 (kappa light chain) | 4 µg/mL |
| ESAM | Endothelial cell adhesion molecule | 199.1 | 4.6 | R&D Systems | MAB4204 | Mouse IgG2b | 10 µg/mL |
| FLNA | Filamin A | 795.9 | 553.5 | Santa Cruz Biotechnology | sc-17749 | Mouse IgG2a (kappa light chain) | 4 µg/mL |
| GJA1 | Gap junction protein alpha 1 | 170.4 | 7.2 | Life Technologies | 13-8300 | Mouse IgG1 | 10 µg/mL |
| LIN7A | Lin 7A, crumbs cell polarity complex | 0.4 | 0.8 | Life Technologies | PA5-30871 | Rabbit polyclonal IgG | 20 µg/mL |
| MPDZ | Multiple PDZ domain crumbs cell polarity complex component | 18.9 | 43.8 | Sigma-Aldrich | HPA020255 | Rabbit polyclonal IgG | 2 µg/mL |
| MYO10 | Myosin X | 29.8 | 15.0 | Life Technologies | PA5-55019 | Rabbit polyclonal IgG | 6 µg/mL |
| PCDH1 | Protocadherin 1 | 61.7 | 15.0 | Life Technologies | PA5-35091 | Rabbit polyclonal IgG | 9.4 µg/mL |
| SLC1A1 | Glutamate transporter | 7.3 | 2.5 | Cell Signaling Technology | 14501 | Rabbit polyclonal IgG | 320 µg/mL |
| SLC2A1 | Glucose transporter 1 | 54.4 | 105.4 | Santa Cruz Biotechnology | sc-377228 | Mouse IgG1 (kappa light chain) | 4 µg/mL |
| SLC3A2 | L-type amino acid transporter | 107.5 | 53.4 | Santa Cruz Biotechnology | sc-376815 | Mouse IgG1 (kappa light chain) | 4 µg/mL |
| SLC5A6 | Sodium-dependent multivitamin transporter | 11.4 | 1.5 | Sigma-Aldrich | HPA036958 | Rabbit polyclonal IgG | 4 μg/mL |
| SLC16A1 | Monocarboxylate transporter 1 | 36.1 | 1.7 | Santa Cruz Biotechnology | sc-365501 | Mouse IgG1 (kappa light chain) | 4 µg/mL |
| SLC19A2 | Thiamine transporter | 2.4 | 2.6 | Life Technologies | PA5-53456 | Rabbit polyclonal IgG | 2 µg/mL |
| TJP1 | Zona occludens-1 | 32.6 | 88.4 | Life Technologies | 61-7300 | Rabbit polyclonal IgG | 2.5 µg/mL |
| VEZT | Vezatin | 28.1 | 11.9 | Life Technologies | PA5-52115 | Rabbit polyclonal IgG | 6 µg/mL |
| ZYX | Zyxin | 201.1 | 35.8 | Life Technologies | PA1-25162 | Rabbit polyclonal IgG | 133 µg/mL |