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. 2017 Dec 12;7:17406. doi: 10.1038/s41598-017-17720-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR/Cas9-mediated disruption of CD38 does not affect signalling proteins known to be involved in RA-induced differentiation. Wild-type and CD38 CRISPR cell lines were cultured for 48 h with 1 μM RA as indicated and whole cell lysate was collected. 25 μg of lysate per lane was run. Western blots of PAGE-resolved lysates from wild-type cells and CRISPR 1, 2, and 3 cell lines were probed for the indicated proteins, where GAPDH is a loading control. Membrane images for each protein are cropped to show only the band of interest.