Fig. 8.
Nav1.1 and Nav1.2 form dimers and co-immunoprecipitate with 14-3-3. a Crosslinking from HEK293 cells transfected with either Nav1.1 or Nav1.2. Crosslinker used was DSS at either 50 or 100 μM for 10 or 20 min. b Co-immunoprecipitation of 14-3-3 with Nav1.1 and Nav1.2 in transfected HEK293 cells. Complex was co-immunoprecipitated using the sodium channel NavPan polyclonal antibody. Both sodium channel and 14-3-3 antibodies were used to reveal the interaction. Bands were shown in all conditions indicating interaction between Nav1.1 or Nav1.2 with 14-3-3. Full blots are presented in Supplementary Fig. 13