Fig. 9.
Neuronal sodium channel, Nav1.1 and Nav1.2 also display coupling at the single-channel level mediated through 14-3-3. a Representative traces of single-channel recordings at −40 mV for Nav1.1 and Nav1.2 expressed in HEK293 cells with or without difopein. The overall amplitudes for single and double openings are represented as mean ± SEM in the gray bars. The dotted lines correspond to single-level openings (O1) and double-level openings (O2), respectively. The number of channels under a patch was estimated by the amplitude and detected by the Clampfit analysis software. b Number of openings at the single or double level in function of time. 400 hundred sweeps were recorded by depolarizing the membrane to −40 mV. The number of openings at level 1 (single or uncoupled dimer) or openings of level 2 (coupled dimer) were plotted in function of time