Figure 2.

Myh9 and Myh10 are the NM2 proteins expressed in the thick ascending limb of the loop of Henle segment. Confocal fluorescence microscopy of the adult mouse kidney sections stained for uromodulin (TAL marker) along with the NM2 proteins individually revealed that Myh9 and Myh10 are the major NM2 proteins in TAL segment of the Loop of Henle tubules. Myh9 (red) is highly expressed in the majority of the uromodulin (green) TAL tubules, with strong localization on the apical membrane (A–C, arrowhead). Myh10 localized to the apical (arrowhead) and basolateral membranes (arrow) in most of the uromodulin‐positive TAL segments (D–F). Myh14 was not expressed in the TAL tubules, although several of the adjacent uromodulin‐negative tubules expressed Myh14 (G–I). Sections were mounted with vectashield containing DAPI to stain the nucleus (blue; C, F & I). Scale bar = 10 μm.