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. 2017 Nov 30;5(23):e13529. doi: 10.14814/phy2.13529

Figure 1.

Figure 1

Cyclic AMP and TUDC inhibit TLC‐induced increases in plasma membrane PKC ε in rat hepatocytes. Hepatocytes were treated with DMSO (Con), 100 μmol/L CPT‐cAMP (cAMP) for 15 min, 10 μmol/L TLC for 25 min, 100 μmol/L CPT‐cAMP + 10 μmol/L TLC for 25 min (upper panel), or 25 μmol/L TUDC for 15 min or 10 μmol/L TLC for 25 min or 25 μmol/L TUDC + 10 μmol/L TLC for 25 min (middle panel). A biotinylation method was used to determine PM‐PKC ε. The relative amount of PM‐PKC ε (based on densitometric analysis) was expressed as a ratio of PM‐PKC ε to E‐cadherin (E‐cad) and is shown in the bar graph. The relative values of PM‐PKC ε are expressed as mean ± SE (n = 4). *Significantly different (P < 0.05) from control values. #Significantly (P < 0.05) different from TLC.