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. 2017 Dec 12;7:17383. doi: 10.1038/s41598-017-17643-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dpo4 binding dynamics to the DNA with adducted dG as templating base in the presence of each nucleotide. (a) Modified DNA sequence used for this single nucleotide incorporation assay (top, Table S1). The label ‘-‘ indicates no nucleotide added and ‘4’ the presence of all four nucleotides. The other lanes contain only the specified nucleotide. Nucleotide incorporation is minimal in the absence of DMSO. In the presence of DMSO dG is incorporated across the adduct. (b) smFRET distributions for Dpo4 binding to unmodified (blue) and B[a]P modified (red) DNA in the presence of 1 mM dNTP as indicated, in the absence (left) and presence (right) of 10% DMSO. When nucleotides are present all histograms fit to single Gaussian distribution.