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. 2017 Dec 12;7:17416. doi: 10.1038/s41598-017-17565-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Apoptosis is significantly increased by 8 hours after progesterone withdrawal in the mouse endometrium. Expression of CC3 was analysed by immunohistochemistry and semi-quantitative histoscoring. (a) Representative photomicrographs of endometrial tissue sections at high power, centred on decidualised stroma and basal stroma. BS = basal stroma, DS = decidualised stroma, Lu = lumen, SE = surface epithelium, SM = smooth muscle. (b) CC3 expression in the surface epithelium (SE; black squares), decidualised stroma (DS; black triangles) and basal stroma (BS; black circles) plotted against time post-progesterone-withdrawal. (c–e) Expression in individual tissues plotted against time post-progesterone withdrawal. Results are presented as mean ± SEM. Significance determined by 1-way ANOVA and Dunnett’s multiple comparisons test (to 0 hours’ progesterone withdrawal). *p < 0.05, **p < 0.01, ****p < 0.0001.