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. 2017 Dec 12;7:17416. doi: 10.1038/s41598-017-17565-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophil abundance is increased by 8 hours and peaks at 24 hours after progesterone withdrawal in the mouse endometrium. Neutrophil abundance was analysed by immunohistochemistry and ImageJ-assisted stereology. (a) Representative photomicrographs of endometrial tissue sections at high power. DS = decidualised stroma, Lu = lumen, SE = surface epithelium. (b) Percentage of endometrial stroma comprised by Ly6G-immunopositive cells (normalised to total cell nuclei) plotted against time post-progesterone-withdrawal. Results are presented as a boxplot constructed from second and third quartiles, with whiskers extending to minimum and maximum values. Significance determined by 1-way ANOVA and Dunnett’s multiple comparisons test (to 0 hours’ progesterone withdrawal). *p < 0.05, ***p < 0.001.