Figure 1.

CCS-D2 forms a stable complex with E,Zn-SOD1^SH both in cells and in vitro. (a) ¹H-¹⁵N NMR spectra of human cells expressing [U-¹⁵N]-labelled WT SOD1 either alone (red) or together with [U-¹⁵N]-labelled CCS-D2 (black). (b) ¹H-¹⁵N NMR spectra of the corresponding cell lysates. (c) In vitro ¹H-¹⁵N NMR spectra of [U-¹⁵N]-labelled WT SOD1 without (orange) and with 1 equivalent (blue) of unlabelled CCS-D2. SOD1 signals experiencing chemical shift changes upon complex formation are labelled in the full spectra (upper panels) and in the detailed views (lower panels). See also Supplementary Figures S1 and S2.