Figure 3.

CCS-D2 interacts with apo-SOD1^SH in vitro. (a) In vitro ¹H-¹⁵N NMR spectra of [U-¹⁵N]-labelled apo-SOD1^SH alone (black) and in the presence of 2 equivalents of unlabelled CCS-D2 (red). The signals used to estimate the molecular reorientation correlation time (τ_m) of apo-SOD1^SH in the complex with CCS-D2 are labelled. (b,c) Detailed views of SOD1 signals (red) experiencing chemical shift changes upon titration with 1 (azure) and 2 (red) equivalents of CCS-D2, showing that apo-SOD1^SH free and in the complex are in the slow exchange regime.