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. 2017 Nov 10;6(12):2984–2997. doi: 10.1002/cam4.1257

Figure 1.

Figure 1

Flow cytometry and sorting strategy for CD5+ B‐cells from human tonsils. The upper panel presents the sorting gates. “Cells” are gated from debris according to forward and side light scatter characteristics; “singlets” are gated according to FSC signal width/height ratio; “B‐cells” are gated based on CD19 fluorescence; a subpopulation of B‐cells (“CD5+ B‐cells”) with a moderate CD5+ staining level; the gating is sequential. To gate on the subpopulation of “CD5+ B‐cells” correctly FMO control was used (see Materials and Methods). CD5 median fluorescence values (MFI) for different lymphocyte populations are as follows: MFI(CD5) T‐cells = 9462, MFI(CD5) B‐cells(general) = 405, MFI(CD5)CD5+B‐cells = 1939. The lower panel expands on CD5+ B‐cell phenotype: unlike B‐CLL the cells of interest are CD23‐low, CD27‐low, CD25‐low, and universally CD38+.