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. 2017 Oct 7;7(3):378–387. doi: 10.1016/j.ijpddr.2017.10.003

Fig. 4.

Fig. 4

Fenofibrate reduces pro-inflammatory mediators in Trypanosoma cruzi-infected mice. IL-6 and TNF-α mRNA levels were analysed by RT-qPCR in total RNA heart extracts of T. cruzi-infected mice or T. cruzi-infected and treated mice (upper panels). The concentration of these cytokines in sera was measured by ELISA (lower panels) (A). NOS2 mRNA and protein levels were determined by RT-qPCR and Western blot with specific primers and antibodies, respectively (B). The RT-qPCR results were normalized against 18S rRNA. Protein levels were normalized against α-actin. Results are expressed as the mean of three independent experiments (seven mice/group) ± SEM. White bar: Uninfected control mice. Black bar: T. cruzi-infected mice. Grey bar: T. cruzi-infected and Fen treated mice. Hatched grey bar: T. cruzi-infected and Fen plus Bzl treated mice **P < 0.001, T. cruzi-infected and treated mice (Fen or Fen plus Bzl) vs. T. cruzi-infected mice; ##P < 0.001, T. cruzi-infected mice vs. uninfected mice.