Figure 2.

Stable gene silencing reduced the chemotactic ability of ND‐01. (a) qRT‐PCR analysis of mcp, aer, cheV, cheB, cheW, cheA, and flgA expression after stable gene silencing compared with the control. Data are presented as the means ±SD, and six independent biological replicates were performed per group. The means of treatments not sharing a common letter are significantly different at p < .05. (b) Chemotaxis capacity of stably silenced ND‐01 to mucus. Data are presented as the means ± SD. (n = 3). Means of treatments not sharing a common letter are significantly different at p < .05, as assessed using one‐way ANOVA followed by Dunnett's test. (c) qRT‐PCR analysis of cheA, cheW, and cheY expression after stable gene silencing compared with the control. Data are presented as the means ± SD, and six independent biological replicates were included for each group. The means of treatments not sharing a common letter are significantly different at p < .05