Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 13;8:2103. doi: 10.1038/s41467-017-02282-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — E2F1 promotes GAr-dependent induction of c-myc. a RT-qPCR data show the relative induction of c-myc-luciferase (Renilla) reporter constructs (indicated in lower panel) following expression of the GAr, as compared to control plasmid (EV). b Relative RT-qPCR values show the induction of c-myc promoter activity from the (−107/+34) luciferase reporter construct using indicated Ova and GAr-Ova (±IRES) cDNA constructs. c Relative RT-qPCR values from the (−107/+34) luciferase reporter constructs carrying either three (Mut1) or five (Mut2) point mutations in the E2F1 consensus binding site (indicated in lower panel). d DNA chromatin IP (ChIP) assay using the (−107/+34) sequence of the c-myc promoter constructs (WT, Mut1 and Mut2) and antibodies against E2F1, E2F2 or E2F3 from cells expressing the GAr or EV. e Western blots showing the E2F1 levels in cells expressing EV, EBNA1 WT, EBNA1 ∆GAr or GAr with actin used as loading control. f RT-qPCR data of the induction of the E2F1 target genes Cyclins E and B following expression of GAr. For RT-qPCR, the values were normalised with GAPDH. The values represent the mean data from three independent experiments with s.d. Significance was calculated using t tests (***p < 0.001 and **p < 0.05)